Document Type : Original Article
Authors
1
Medical Physiology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
2
Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
3
Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
4
Pathology Department, Faculty of Medicine, Mansoura University, Egypt.
5
Endocrinology unit, Internal Medicine Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
6
Clinical Pharmacology Department, Faculty of Medicine, Ain Shams university, Egypt.
7
Medical Biochemistry and Molecular Biology Department Faculty of Medicine, Mansoura University, Mansoura, Egypt
8
Anatomy and Embryology Department, Faculty of Medicine, Portsaid University Egypt
9
Medical Physiology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.
10
Cardiology Department, Faculty of Medicine, Suez University, Egypt.
11
Zoology department, Faculty of Science, Menoufia University.
Abstract
Background: Cardiac inflammation, oxidative stress, and myocardial metabolic disruption are among the pathologic abnormalities that can result from diabetic cardiomyopathy (DCM), an independent diabetic cardiac disease. A new myokine called irisin has preventive properties against heart conditions.
Objective: to demonstrate the potential underlying processes and cardiovascular protective impact of Irisin in diabetic cardiomyopathy.
Material and methods: DCM, DCM+Irisin, and control (10/group) were the three groups into which thirty male albino rats were divided. After eight weeks, assessments of the following were made: LVW/ tibial length, serum glucose, serum insulin, HOMA-IR index, serum glycosylated Hb A1c, serum cholesterol, serum triglyceride, serum cTnI, serum LDH, serum CK-MB, cardiac MDA, cardiac SOD, cardiac TNF-α, cardiac IL-6, cardiac IL-10, cardiac HMGB1 gene expression, cardiac TLR4 gene expression and cardiac NF-kB gene expression. Furthermore, histological and immunohistochemical examinations of the heart and aorta were carried out.
Results: The measured LVW/ tibial length, serum glucose, serum Insulin, HOMA-IR index, serum glycosylated Hb A1c, serum cholesterol, serum triglyceride, serum cTnI, serum LDH, serum CK-MB, cardiac MDA, cardiac TNF-α, cardiac IL-6, cardiac gene expression of HMGB1, TLR4 and NF-kB, were all markedly raised in DCM group compared to control, while the DCM group's cardiac SOD, and cardiac IL-10 were substantially lower than those of the control. Additionally there was dramatically downregulated cardiac and aortic NF-kB immunoreaction of DCM group compared to control. Irisin significantly mitigated diabetic cardiomyopathy induced changes.
Conclusion: Irisin protects against DCM by downregulating the cardiac HMGB1/TLR4/NF-kB signaling pathway and displaying lipid-lowering, anti-inflammatory and antioxidant impacts.
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