Determining the Prevalence of Primary and Secondary Central Line Associated Blood Stream Infection (CLABSI) at the Tertiary Care Hospital

Sadek, Refat 1; Halawa, Marwa; Rohaeim, Cherihan; Sadek, Mona

doi:10.21608/muj.2025.369899.1218

Determining the Prevalence of Primary and Secondary Central Line Associated Blood Stream Infection (CLABSI) at the Tertiary Care Hospital

Articles in Press

Document Type : Original Article

Authors

¹ professor of microbiology and immunology faculty of medicine port said university

² Microbiology &immunology dept Portsaid faculty of Medicine

³ Lecturer of Microbiology Portsaid faculty of medicine

⁴ Lecturer of Clinical Pathology , Mansoura Faculty of Medicine

10.21608/muj.2025.369899.1218

Abstract

As a serious side effect of infections (such as meningitis or pneumonia), during surgery (particularly when mucous membranes are involved, like in the gastrointestinal tract), or when catheters and other foreign objects get into the arteries or veins (including during intravenous drug abuse), bacteria can enter the bloodstream. Content and Procedures: This study looks back at all hospitalized patients who had clinically significant bacteraemia between December 1, 2023, and August 31, 2024. Adult and pediatric BacT/ALERT blood culture bottles were inoculated with 5–10 ml of adult and 2–3 ml of pediatric patients. At 37°C, they were subsequently continuously incubated aerobically. Results: The results showed that only 176(20.1%) of the blood culture broths were bacterially culture-positive. Among patients aged 6–17 years, culture positivity was lowest (12.9%), while it was highest among neonates (less than 1 month; 23.8%). The cultural positivity of male and female participants did not differ significantly (p = 0.96). Most identified bacteria were gram-negative enteric (57.3%), including Klebsiella aerogenes (41.1%) and Escherichia coli (8.2%). Sixty-one percent were sensitive to piperacillin-tazobactam, seventy-seven percent were sensitive to gentamicin, and eighty-one percent for amikacin. The most frequent Gram-positive bacterium that was isolated (26.3%) was S. aureus. Of the S. aureus isolates, 86% were resistant to methicillin. 35% had imipenem and meropenem sensitivity. 33% of gram-positive isolates, including MRSA, were vancomycin sensitive. Conclusion: Most gram-negative bacteria were recovered from BSI cases. Good sensitivity to ceftazidime, imipenem, ciprofloxacin/ofloxacin, gentamicin, and meropenem was shown by the isolates.

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